Cell aggregation: spheroid formation with hanging drop technique
INTRODUCTION The cell aggregation is the combination of two cellular functions: cell-to-cell recognition and cell adhesion. In comparison with bidimensional […]
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3D bioprinting of human dermal fibroblast cell-laden photocurable GelMA hydrogels
INTRODUCTION Gelatin hydrogels have attracted significant attention in the field of regenerative medicine, and more specifically, in their use as […]
REG4Life working protocol inside a biological cabinet
INTRODUCTION Working in sterile conditions is key for culturing our scaffolds in aseptic conditions and avoiding contamination. In a 3D […]
Protocol for 3D bioprinting of cell-laden Colbioink scaffolds
INTRODUCTION Type I collagen is the most abundant type of collagen in the human body, as a major structural matrix […]
In-hydrogel chondrogenic differentiation
INTRODUCTION Hydrogels are at the forefront of 3D bioprinting and tissue engineering. However, the wide variety of choices available, and […]
Protocol for 3D bioprinting of Fibercoll-FlexN bioinks
INTRODUCTION Collagen is the most abundant structural protein in the extracellular matrix (ECM), accounting for 30% of the total body […]
Protocol for coaxial 3D printing of sodium alginate
INTRODUCTION Alginate is a FDA-approved natural polysaccharide extracted from brown algae with multiple applications in the food, cosmetic, and pharmaceutical […]