+20 Human articular chondrocytes were isolated as previously described (Lopez-Ruiz et al., 2013) Chondrocytes were grown in Dulbecco’s modified eagle’s medium (DMEM) – high glucose (Sigma) supplemented with 10% fetal bovine serum (Gibco), 50 microg/microL of l-ascorbic acid 2-phosphate (Sigma), 1% penicillin-streptomycin (Sigma), and 1% ITS (Gibco) in a 25-cm2 […]
Colagen II
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