00 INTRODUCTION Hematoxylin-Eosin stain (H&E stain) is one of the main tissue stains used in histology. It is the most widely used stain in medical diagnosis and is often the gold standard in histopathology. H&E is the combination of two histological stains: hematoxylin and eosin. Nuclei stained with hematoxylin display […]
Biological tests
14 posts
00 Introduction Thermoplastic polyurethanes (TPU) are a category of materials broadly used for biomedical purposes thanks to their biocompatibility, elasticity and strength. Among the different manufacturing techniques, 3D printing technology has attracted significant attention for the production personalized TPU-based medical devices over the last decade. The preference of this technique […]
+10 INTRODUCTION Alginate, also known as alginic acid, is a natural polysaccharide found in brown algae. It has a linear copolymer structure comprised of homopolymeric blocks of (1→4)-linked β-D-mannuronate and α-L-guluronate residues. Given the similarity of its chemical structure to polysaccharides naturally present in the extracellular matrix of human tissues, […]
00 INTRODUCTION Gelatin hydrogels have attracted significant attention in the field of regenerative medicine, and more specifically, in their use as wound dressings and artificial tissue replacements. Among the different photo-crosslinkable gelatin materials, gelatin methacryloyl (GelMA) hydrogels display a series of advantages, such as good biocompatibility (including biosafety and biological […]
+30 INTRODUCTION Type I collagen is the most abundant type of collagen in the human body, as a major structural matrix protein in skin and many other tissues including bone, tendon and fibrous connective tissues. ColBioink is a sterile solution of highly purified collagen type I from porcine origin that […]
+30 INTRODUCTION Hydrogels are at the forefront of 3D bioprinting and tissue engineering. However, the wide variety of choices available, and the diverse physicochemical nature of the biomaterials comprising the hydrogels, complicate the selection of the most adequate formulation for each specific application. The purpose of this method is to […]
+10 INTRODUCTION Collagen is the most abundant structural protein in the extracellular matrix (ECM), accounting for 30% of the total body protein content in humans. Amongst the 28 types of collagen that have been already identified, collagen type I is the most abundant by far, playing its most prominent functional […]
+20 Facilan™ PCL 100 Filament (MW = 50000 g/mol) was used as 3D printing filament. The scaffolds were designed with the software Regemat3D Designer v1.4.4 and printed using a Regemat3D Bio V1® bioprinter (REG4Life, REGEMAT 3D, Granada, Spain) equipped with a glass bed and a 0.4 mm diameter nozzle. The […]
+100 Introduction: Injuries to the knee meniscus commonly lead to osteoarthritis. Current therapies for meniscus regeneration, including meniscectomies and scaffold implantation, do not achieve complete functional tissue regeneration. This has led to increased interest in cell and gene therapies and tissue engineering approaches to meniscus regeneration. The implantation of a […]
+30 In this method we describe the parameters to FDM bioprint scaffolds for Bone regeneration Composed by of 80% PCL that had a MW 80,000 (Polysciences Europe, Hirschberg an der Bergstraße, Germany) and 20% of the synthetic biphasic calcium phosphate bone substitute material (BCP) (synprint, ScientiFY GmbH, Berlin, Germany) with […]