00 INTRODUCTION Hematoxylin-Eosin stain (H&E stain) is one of the main tissue stains used in histology. It is the most widely used stain in medical diagnosis and is often the gold standard in histopathology. H&E is the combination of two histological stains: hematoxylin and eosin. Nuclei stained with hematoxylin display […]
Drug discovery and development
11 posts
+10 INTRODUCTION STABLE INX© (BIOINX©) is a synthetic, shear thinning, cell-interactive and non-biodegradable hydrogel bioink which has been specifically designed for the fabrication of scaffolds for tissue engineering. Due to its shear thinning behavior, it allows for an easy printing process. It exhibits a low viscosity at high shear rates, […]
+10 INTRODUCTION Alginate, also known as alginic acid, is a natural polysaccharide found in brown algae. It has a linear copolymer structure comprised of homopolymeric blocks of (1→4)-linked β-D-mannuronate and α-L-guluronate residues. Given the similarity of its chemical structure to polysaccharides naturally present in the extracellular matrix of human tissues, […]
00 INTRODUCTION The cell aggregation is the combination of two cellular functions: cell-to-cell recognition and cell adhesion. In comparison with bidimensional (2D) cell culture, the three-dimensional (3D) cell aggregates provide functional microtissues that mimic the native tissue conditions of growth and adhesion. The aggregation can be done with cells from […]
+20 In the picture: 👀 99% of cell viability with ColBioink formulation. This material is a native Type I collagen formulation that comes from porcine origin and is in a “ready to print format”. It also ensures that the formed structures can be safely grafted without immune rejection and used […]
00 “Next generation gelatin-based bio-ink.” Introduction X EASYGEL INX© is a gelatin-based shear thinning, cell-interactive ECM mimicking ink. X EASYGEL INX combines all the benefits of conventional gelatin/gel-MA based inks in combination with a highly improved printing process thanks to its shear thinning behavior, as schematically presented in Figure 1a. […]
+10 Hydroxypropyl methylcellulose METHOCEL ™ K4M (Colorcon Ltd., UK) was used as hydrophilic polymer to prepare drug-loaded hydrogels for IVF. Anhydrous theophylline (Acofarma, Spain) has been used as a model drug. HPMC (1% w/v) gel was prepared by adding powdered HPMC into 100 ml of hot water (90 °C) and […]
+10 Introduction Material extrusion is an additive manufacturing technique that uses a continuous filament of thermoplastic or composite material to construct 3D parts. Filament-based material extrusion is the most common practice in the 3D printing community. However, it’s not necessary for extrusion to only work with filament. In fact, pellet […]
+20 Introduction Liposomes are spherical vesicles consisting of one or more concentric phospholipid bilayers enclosing an aqueous core. Nontoxic and biodegradable liposomes are useful tools to package both hydrophilic and hydrophobic biomolecules (including therapeutic agents) that may be further incorporated into target cells to elicit functional changes. Extensive research is […]
+30 3cc X- gel-Ma INX ready-to-use syringes were loaded and the bioprinted using the V1 REGEMAT3D bioprinter, equipped with the REGEMAT3D Designer Version 1.5, REGEMAT3D, Granada, Spain. Grids having a 10mmx10mm in width and length and 1mmx1mm as pore size were printed directly on the glass for testing purposes. The […]