00 INTRODUCTION Hematoxylin-Eosin stain (H&E stain) is one of the main tissue stains used in histology. It is the most widely used stain in medical diagnosis and is often the gold standard in histopathology. H&E is the combination of two histological stains: hematoxylin and eosin. Nuclei stained with hematoxylin display […]
Application
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00 Introduction Thermoplastic polyurethanes (TPU) are a category of materials broadly used for biomedical purposes thanks to their biocompatibility, elasticity and strength. Among the different manufacturing techniques, 3D printing technology has attracted significant attention for the production personalized TPU-based medical devices over the last decade. The preference of this technique […]
+10 INTRODUCTION STABLE INX© (BIOINX©) is a synthetic, shear thinning, cell-interactive and non-biodegradable hydrogel bioink which has been specifically designed for the fabrication of scaffolds for tissue engineering. Due to its shear thinning behavior, it allows for an easy printing process. It exhibits a low viscosity at high shear rates, […]
+10 INTRODUCTION Alginate, also known as alginic acid, is a natural polysaccharide found in brown algae. It has a linear copolymer structure comprised of homopolymeric blocks of (1→4)-linked β-D-mannuronate and α-L-guluronate residues. Given the similarity of its chemical structure to polysaccharides naturally present in the extracellular matrix of human tissues, […]
00 INTRODUCTION The cell aggregation is the combination of two cellular functions: cell-to-cell recognition and cell adhesion. In comparison with bidimensional (2D) cell culture, the three-dimensional (3D) cell aggregates provide functional microtissues that mimic the native tissue conditions of growth and adhesion. The aggregation can be done with cells from […]
00 INTRODUCTION Gelatin hydrogels have attracted significant attention in the field of regenerative medicine, and more specifically, in their use as wound dressings and artificial tissue replacements. Among the different photo-crosslinkable gelatin materials, gelatin methacryloyl (GelMA) hydrogels display a series of advantages, such as good biocompatibility (including biosafety and biological […]
00 INTRODUCTION Working in sterile conditions is key for culturing our scaffolds in aseptic conditions and avoiding contamination. In a 3D Bioprinting protocol we have many sources of contamination, the biomaterial, the cells itself or the 3D bioprinting protocol. The scaffolds with or without cells have the same requirements in […]
+30 INTRODUCTION Hydrogels are at the forefront of 3D bioprinting and tissue engineering. However, the wide variety of choices available, and the diverse physicochemical nature of the biomaterials comprising the hydrogels, complicate the selection of the most adequate formulation for each specific application. The purpose of this method is to […]
+10 INTRODUCTION Collagen is the most abundant structural protein in the extracellular matrix (ECM), accounting for 30% of the total body protein content in humans. Amongst the 28 types of collagen that have been already identified, collagen type I is the most abundant by far, playing its most prominent functional […]
+10 INTRODUCTION Alginate is a FDA-approved natural polysaccharide extracted from brown algae with multiple applications in the food, cosmetic, and pharmaceutical industries. Its outstanding features including high biocompatibility, ease of gelation, tailorable rheological properties and degradation kinetics make it ideal for 3D bioprinting applications. Alginate hydrogels can be ionically crosslinked […]