Isolation of RNA from cell-laden hydrogel-thermoplastic hybrid tissue-engineered constructs
INTRODUCTION Tissue engineering aims for assembling functional constructs that restore, maintain, or improve damaged tissues or whole organs. In the […]
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Protocol for the fabrication of VitroINK 3D xeno-free scaffolds through mechanical extrusion-based 3D printing
INTRODUCTION Synthetic hydrogels are chemically defined animal component and pathogen-free hydrogels with tailored biological, mechanical and rheological properties for 3D […]
3D printing of highly flexible scaffolds using Flexdym
Introduction FlexdymTM is a block co-polymer belonging to the styrene-ethylene-butylene-styrene (SEBS) family of thermoplastic elastomers which is specifically tailored for […]
Protocol for 3D printing of EASYGEL INX© scaffolds for tissue engineering
INTRODUCTION EASYGEL INX© X100 is a gelatin-based, shear thinning, cell-interactive, extracellular matrix (ECM)-mimicking ink. It provides all the benefits of […]
Protocol for Hematoxylin-Eosin staining of tissue sections
INTRODUCTION Hematoxylin-Eosin stain (H&E stain) is one of the main tissue stains used in histology. It is the most widely […]
Culture of astrocytes on flexible and electrically conductive 3D printed scaffolds
Introduction Thermoplastic polyurethanes (TPU) are a category of materials broadly used for biomedical purposes thanks to their biocompatibility, elasticity and […]
Protocol for 3D printing of STABLE INX© scaffolds for tissue engineering
INTRODUCTION STABLE INX© (BIOINX©) is a synthetic, shear thinning, cell-interactive and non-biodegradable hydrogel bioink which has been specifically designed for […]