00 INTRODUCTION GEL-MA INX© is a gelatin-based bioink that exhibits excellent biomimicry given it derives from natural collagen, the main component of the extracellular matrix. GEL-MA INX© is based on the functionalisation of gelatin by covalent attachment of methacryloyl moieties to its amine groups, yielding gelatin-methacrylamide (GEL-MA). GEL-MA INX© is […]
Drug discovery and development
14 posts
00 INTRODUCTION Tissue engineering aims for assembling functional constructs that restore, maintain, or improve damaged tissues or whole organs. In the quest for the optimal tissue-engineered solution per specific application, the employment of gene expression analysis techniques is commonly desired. A pre-requisite for gene expression analysis is the isolation of […]
+10 INTRODUCTION EASYGEL INX© X100 is a gelatin-based, shear thinning, cell-interactive, extracellular matrix (ECM)-mimicking ink. It provides all the benefits of gelatin with the asset of an unique shear thinning behaviour which, in contrast with many gelatin formulations, allows easy printing at 37 °C. Based on gelatin derived from natural […]
+10 INTRODUCTION Hematoxylin-Eosin stain (H&E stain) is one of the main tissue stains used in histology. It is the most widely used stain in medical diagnosis and is often the gold standard in histopathology. H&E is the combination of two histological stains: hematoxylin and eosin. Nuclei stained with hematoxylin display […]
+10 INTRODUCTION STABLE INX© (BIOINX©) is a synthetic, shear thinning, cell-interactive and non-biodegradable hydrogel bioink which has been specifically designed for the fabrication of scaffolds for tissue engineering. Due to its shear thinning behavior, it allows for an easy printing process. It exhibits a low viscosity at high shear rates, […]
+10 INTRODUCTION Alginate, also known as alginic acid, is a natural polysaccharide found in brown algae. It has a linear copolymer structure comprised of homopolymeric blocks of (1→4)-linked β-D-mannuronate and α-L-guluronate residues. Given the similarity of its chemical structure to polysaccharides naturally present in the extracellular matrix of human tissues, […]
00 INTRODUCTION The cell aggregation is the combination of two cellular functions: cell-to-cell recognition and cell adhesion. In comparison with bidimensional (2D) cell culture, the three-dimensional (3D) cell aggregates provide functional microtissues that mimic the native tissue conditions of growth and adhesion. The aggregation can be done with cells from […]
+20 In the picture: 👀 99% of cell viability with ColBioink formulation. This material is a native Type I collagen formulation that comes from porcine origin and is in a “ready to print format”. It also ensures that the formed structures can be safely grafted without immune rejection and used […]
00 “Next generation gelatin-based bio-ink.” Introduction X EASYGEL INX© is a gelatin-based shear thinning, cell-interactive ECM mimicking ink. X EASYGEL INX combines all the benefits of conventional gelatin/gel-MA based inks in combination with a highly improved printing process thanks to its shear thinning behavior, as schematically presented in Figure 1a. […]
+10 Hydroxypropyl methylcellulose METHOCEL ™ K4M (Colorcon Ltd., UK) was used as hydrophilic polymer to prepare drug-loaded hydrogels for IVF. Anhydrous theophylline (Acofarma, Spain) has been used as a model drug. HPMC (1% w/v) gel was prepared by adding powdered HPMC into 100 ml of hot water (90 °C) and […]