+100 Introduction: Injuries to the knee meniscus commonly lead to osteoarthritis. Current therapies for meniscus regeneration, including meniscectomies and scaffold implantation, do not achieve complete functional tissue regeneration. This has led to increased interest in cell and gene therapies and tissue engineering approaches to meniscus regeneration. The implantation of a […]
Biological tests
16 posts
+30 In this method we describe the parameters to FDM bioprint scaffolds for Bone regeneration Composed by of 80% PCL that had a MW 80,000 (Polysciences Europe, Hirschberg an der Bergstraße, Germany) and 20% of the synthetic biphasic calcium phosphate bone substitute material (BCP) (synprint, ScientiFY GmbH, Berlin, Germany) with […]
+20 Introduction Fibrin is a degradable, resorbable biopolymer that naturally occurs in the wound healing process at the vascular level. It is formed from fibrinogen, a glycoprotein synthesised by the liver and found in blood plasma. When tissue damage occurs and results in bleeding, fibrinogen is converted into fibrin at […]
+20 Human articular chondrocytes were isolated as previously described (Lopez-Ruiz et al., 2013) Chondrocytes were grown in Dulbecco’s modified eagle’s medium (DMEM) – high glucose (Sigma) supplemented with 10% fetal bovine serum (Gibco), 50 microg/microL of l-ascorbic acid 2-phosphate (Sigma), 1% penicillin-streptomycin (Sigma), and 1% ITS (Gibco) in a 25-cm2 […]
+10 Introduction 3D in vitro models offer a better representation of in vivo conditions, which is translated into differences in cellular morphology, metabolism and functions when compared with cultures in monolayer. To measure such differences, a plethora of indicators can be used; with the alamarBlue assay, levels of oxidation during […]
+30 Introduction To date, most cell-based assays use traditional 2D monolayer cells cultured on flat and rigid substrates. However, these models are unable to reproduce the architecture, mechanical and functional properties inherent to native tissues, thus hampering the translation of research results into clinics. For these reasons, 3D cell culture […]