+10 3D printed systems production: Printfills (3D printed systems) were manufactured employing a bioprinter (Regemat 3D S.L., Spain) which combines FDM and IVF techniques. Two different batches of printfills were designed by the software Regemat 3D DESIGNER. First batch contained free CPT, whereas the second one CPT encapsulated within […]
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+30 The printing process of FILAMENT-Oss is carried out by a Fused Filament Fabrication process (FFF/FDM). As any other Additive Manufacturing technique, the FFF creates a physical 3D object from a digital model (usually a CAD representation) which is sliced in layers at consecutive height levels. The process requires of […]
+30 In this method we describe the parameters to FDM bioprint scaffolds for Bone regeneration Composed by of 80% PCL that had a MW 80,000 (Polysciences Europe, Hirschberg an der Bergstraße, Germany) and 20% of the synthetic biphasic calcium phosphate bone substitute material (BCP) (synprint, ScientiFY GmbH, Berlin, Germany) with […]
+30 CNF Nanocellulose gels (2 wt %) were used as inks for 3D printing. The 3D printing was performed with a Regemat3D bioprinter (version 1.0), equipped with the Regemat3D Designer (version 1.8, Regemat3D, Granada, Spain). Grids having a diameter of 20 mm and a height of 2 mm were printed […]
+70 This method describes a robust and reproducible method for ready isolation and expansion of ovine Wharton’s jelly-derived MSC (oWJ-MSC) from umbilical cord (UC) for use in non-clinical research to support proof-of-concept studies addressing safety, efficacy, and the study of mechanisms of action (MoA) of novel MSC-based treatments. The protocol […]
+20 Human articular chondrocytes were isolated as previously described (Lopez-Ruiz et al., 2013) Chondrocytes were grown in Dulbecco’s modified eagle’s medium (DMEM) – high glucose (Sigma) supplemented with 10% fetal bovine serum (Gibco), 50 microg/microL of l-ascorbic acid 2-phosphate (Sigma), 1% penicillin-streptomycin (Sigma), and 1% ITS (Gibco) in a 25-cm2 […]