3cc X- gel-Ma INX ready-to-use syringes were loaded and the bioprinted using the V1 REGEMAT3D bioprinter, equipped with the REGEMAT3D Designer Version 1.5, REGEMAT3D, Granada, Spain. Grids having a 10mmx10mm in width and length and 1mmx1mm as pore size were printed directly on the glass for testing purposes. The bioink was kept at room temperature (25ºC) 12hs before. The nozzle of the syringe was 0.41mm (inner diameter). The flow speed was 1.75 mm/s. The intensity of the UV light was 100% and the UV light curing speed was 0.5mm/s.
Product Overview
X gel-Ma INX© is a gelatin based ink exhibiting ideal extra cellular matrix mimicking properties as it is derived from natural collagen, the main component of the natural extra cellular matrix. X gel-Ma INX is based on gelatin-methacryloyl, originally developed by our business developer in 20001 . Gel-MA derived from gelatin type B shows ideal properties as it is cell-interactive due to the presence of RGD motifs in its backbone. It is biodegradable and can be remodeled by cells. It can be printed by taking advantage of its temperature dependent physical gelation behavior. Whereas it becomes physiologically stable after photo-crosslinking, thereby forming a non-soluble network. As a result of all these beneficial properties gel-MA has emerged to be one of the gold standards in the field of tissue engineering and biofabrication2 . With X gel-MA INX, the benefits of gel-MA are combined with a high degree of reproducibility and ease of use by offering the material in ready-to-print cartridges, which are subjected to strict quality control.
With X gel-MA INX, the benefits of gel-MA are combined with a high degree of reproducibility and ease of use by offering the material in ready-to-print cartridges, which are subjected to strict quality control.
Physical Characteristics
X gel-MA INX is a transparent gel at room temperature, which turns into a liquid after heating above 30°C. Some physical characteristics of X gel-MAINX are listed in Table 1.
X gel-MA INX is a photo-curable material as shown in Figure 2. This means that after curing, the material becomes physiologically stable, while still being biodegradable. At the end of the irradiation process, the ink exhibits storage moduli in the range of 10 to 15 kPa, meaning that the material exhibits sufficient mechanical integrity to maintain shape retention, while being suitable for a whole range of soft tissue applications.
Storage and loss moduli of X gel-MA INX© recorded via a rheometer during UV irradiation
High Biocompatibility
X gel-Ma INX© is a very versatile bio-ink from a biological perspective due to its strong resemblance to the natural extracellular matrix, and cell interactive behavior. As a result, it has already shown compatibility for a whole range of tissues including adipose tissue, blood vessels, brain tissue, cartilage tissue, bone tissue, connective tissue and ocular tissue.
Biological application potential of X gel-MA INX©
Key Properties
- Biocompatibility: supports cell adhesion and proliferation
- Biodegradability: enables cellular remoddeling of the printed matrix
- Easy Handling: Delivered in a Ready-to-use cartridge
- UV-Curable: Efficient UV-based crosslinking
- Reproducibility: Production under strict quality control
X-gelMa-INX is provided as a clear hydrogel in a prefilled cartridge of 3 ml · Can be printed immediately after heating · UV-crosslinkable.
STORAGE AND HANDLING ·
PH: 7 – 8 · Viscosity: 0.05 – 1000 Pa.s @ 25 °C · Stable for 3 months at 4 – 8 °C
Bibliography:
- Van Den Bulcke, A. I. et al. Structural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels. Biomacromolecules 1, 31–38 (2000).
- Van Hoorick, J. et al. (Photo-)crosslinkable gelatin derivatives for biofabrication applications. Acta Biomater. 97, 46–73 (2019).
| NUMBER | CATEGORY | PRODUCT | AMOUNT |
| 1 | Biomaterials | X-gelMa-INX | 3cc ready-to-use syringe |
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