Materials
• Claro™ BG800 Bionk kit (REGEMAT 3D, RGMB025 – 027)*
• 5 cc syringe (REGEMAT 3D, xxxx)*
• Blue syringe tips (details)
• Phosphate Buffer Saline (PBS)
Equipment
• Dual Tempered Syringe (REGEMAT 3D, XXXX)*
• UV light tool (450 nm, REGEMAT 3D, XXXX)
• BIO V1 or REG4Life 3D printer*
• Laminar flow cabinet*
1. Preparation and loading
1.1 Turn on laminar flow cabinet if sterile printing conditions are required. Let the air flow run for 5-
10 minutes while cleaning the surface with EtOH 70% and preparing the required materials;
1.2 Turn on the printer, open the R3D Studio software and connect the printer using the power
button on the top right of the screen;
1.3 Select the Platform menu > Petri dish.
Note: Remember Petri Dish should not be attached until after auto calibration.
1.4 From the Tools Selection menu, choose the print head (Tool 0 and T2) and the tools required
(Syringe 5 cc > Dual Tempered Syringe; UV light > 450 nm, respectively).
Note: The dual tempered syringe tool only admits for its position to be in T0 and does not allow for any
tool to be in T1 position.
1.5 Select the nozzle diameter and printing temperature. Click update tools to save the information.
1.6 Calibrate the required tools from the Tools Selection menu > Tool 0 > Autocalibrate and Tools
Selection menu > Tool 2 > Autocalibrate.
Note: To perform autocalibration you can use an empty syringe so that the bioink is maintained at a
constant temperature.
1.7 To perform the fine calibration, change the type of print bed to a Petri dish usignthe attatcment
and click on Fine calibration to adjust the nozzle’s height.
1.8 Pre-heat the print head to the desired temperature: 16 ºC.
Note: Printing temperatures vary according to GelMa concentration in the bioink, standarized
concentrations and printing temperatures can be seen here. Mind specific temperature conditions
inside the lab may vary printing temperature.
Read the entire protocol here: BIOPRINTING-PROTOCOLS-CLARO-BG800(1)
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